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ELK Biotechnology

ELK9615

Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit

Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit

Sensitivity

Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit

Overview

Product name: Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit
Reactivity: Mouse
Alternative Names: MGC133365; PSAP; PSPA; SFTP1; SFTPA1
Assay Type: Sandwich
Sensitivity: 0.19 ng/mL
Standard: 20 ng/mL
Detection Range: 0.31-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.954 1.866
10.00 1.612 1.524
5.00 1.251 1.163
2.50 0.975 0.887
1.25 0.617 0.529
0.63 0.368 0.280
0.32 0.226 0.138
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SP-A1 and the recovery rates were calculated by comparing the measured value to the expected amount of SP-A1 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 79-93% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SP-A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 92-103% 90-99% 89-102%
EDTA plasma(n=5) 87-102% 85-94% 87-101% 95-102%
Heparin plasma(n=5) 89-98% 95-103% 97-103% 82-94%
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