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ELK Biotechnology

ELK9776

Mouse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Mouse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Sensitivity

Mouse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Overview

Product name: Mouse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Reactivity: Mouse
Alternative Names: TBARS; Thiobarbituric Acid Reactive Substance
Assay Type: Competitive Inhibition
Sensitivity: 3.55 nmol/ml
Standard: 400 nmol/ml
Detection Range: 6.25-400 nmol/ml
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 2h
Research Area: Metabolic pathway;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TBARS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 0.197 —
200.00 0.332 —
100.00 0.587 —
50.00 0.842 —
25.00 1.157 —
12.50 1.585 —
6.25 1.624 —
0.00 2.226 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TBARS and the recovery rates were calculated by comparing the measured value to the expected amount of TBARS in samples.
Matrix Recovery range Average
serum(n=5) 78-95% 86%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 94-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TBARS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-101% 88-102% 79-92% 85-96%
EDTA plasma(n=5) 85-93% 89-97% 95-102% 87-101%
Heparin plasma(n=5) 89-102% 88-103% 83-94% 92-105%
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