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ELK Biotechnology

ELK9710

Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit

Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit

Sensitivity

Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit

Overview

Product name: Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
Reactivity: Mouse
Alternative Names: TPO Ab; TPO-Ab
Assay Type: Competitive Inhibition
Sensitivity: 1.25 IU/mL
Standard: 80 IU/mL
Detection Range: 1.25-80 IU/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TPO-Ab protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
80.00 0.217 —
40.00 0.332 —
20.00 0.557 —
10.00 0.899 —
5.00 1.253 —
2.50 1.596 —
1.25 1.877 —
0.00 2.375 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TPO-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TPO-Ab in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 94-106% 91-101% 80-92%
EDTA plasma(n=5) 86-102% 87-103% 89-101% 85-96%
Heparin plasma(n=5) 95-104% 82-90% 89-102% 89-102%
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