NAD/NADH Assay Kit-WST, 100 tests

Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD⁺ levels can be determined by subtracting NADH levels from independently measured total NAD⁺/NADH levels.

Principle
NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio.

Measurement of NAD⁺ and NADH

Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD⁺levels can be determined by subtracting NADH levels from independently measured total NAD⁺/NADH levels.