Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human BIK. AA range:18-67
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
30 25kD
GeneID（Human）:
638
Human Swiss-Prot No:
Q13323
Cellular localization:
Endomembrane system; Single-pass membrane protein. Mitochondrion membrane ; Single-pass membrane protein . Around the nuclear envelope, and in cytoplasmic membranes.
Background:
The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011],
Western Blot analysis of various cells using NBK Polyclonal Antibody diluted at 1:500
Immunofluorescence analysis of HUVEC cells, using BIK Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using BIK Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC/COLO205, using BIK Antibody. The lane on the right is blocked with the synthesized peptide.