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ELK Biotechnology
SKU(재고 관리 코드):ES7978
Nek9 (phospho Thr210) Rabbit Polyclonal Antibody
Nek9 (phospho Thr210) Rabbit Polyclonal Antibody
Nek9 (phospho Thr210) Rabbit Polyclonal Antibody
Overview
| Product name: | Nek9 (phospho Thr210) rabbit pAb |
| Alternative Names: | NEK9; KIAA1995; NEK8; NERCC; Serine/threonine-protein kinase Nek9; Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related protein kinase 9; NimA-related kinase 8; Nek8 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NEK9 around the phosphorylation site of Thr210. AA range:176-225 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 110kD |
| GeneID(Human): | 91754 |
| Human Swiss-Prot No: | Q8TD19 |
| Cellular localization: | Cytoplasm . Nucleus . |
| Background: | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NEK9 (Phospho-Thr210) Antibody -
Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.
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