Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from the Internal region of human NRP1. AA range:321-370
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
100kD
GeneID（Human）:
8829
Human Swiss-Prot No:
O14786
Cellular localization:
[Isoform 2]: Secreted .; Mitochondrion membrane ; Single-pass type I membrane protein . Cell membrane ; Single-pass type I membrane protein . Cytoplasm .
Background:
This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011],
Western Blot analysis of 293 cells using Neuropilin Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
Western blot analysis of lysate from 293 cells, using NRP1 Antibody.