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Dima Biotech

SKU(재고 관리 코드):PME100007

Human FLT3 Protein, hFc-His Tag

Human FLT3 Protein, hFc-His Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

Flt-3;Flk-2;STK-1;CD135;FLK2;FLT-3

DESCRIPTION

Recombinant human FLT3 protein with C-terminal human Fc and 6×His tag

DELIVERY

In Stock

UNIPROT ID

P36888

EXPRESSION HOST

HEK293

TAG

C-Human Fc and 6×His Tag

MOLECULAR CHARACTERIZATION

FLT3(Asn27-Ser543) hFc(Glu99-Ala330) 6×His tag

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 125-130 kDa after removal of the signal peptide.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

USAGE

Research use only

IMAGES

sp-PME100007 FLT3 hFc His PAGE 300

Figure 1. Human FLT3, hFc-His Tag on SDS-PAGE under reducing condition.

elisa-PME100007 FLT3 hFc His ELISA Fig2

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human FLT3LG, mFc-His tagged protein (PME100033) can bind Human FLT3, hFc-His tagged protein (PME100007) in a linear range of 0.128-10.02 ng/ml.

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