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Dima Biotech

SKU(재고 관리 코드):PME100009

Human SIRPα Protein, hFc-His Tag

Human SIRPα Protein, hFc-His Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

SHPS1;SIRPA;CD172A;BIT;MFR;MYD1;P84;PTPNS

DESCRIPTION

Recombinant Human SIRPa with C-terminal human Fc and 6×His tag

DELIVERY

In Stock

UNIPROT ID

P78324

EXPRESSION HOST

HEK293

TAG

C-Human Fc and 6×His Tag

MOLECULAR CHARACTERIZATION

SIRPa(Glu31-Tyr373) hFc(Glu99-Ala330) 6×His tag

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

USAGE

Research use only

IMAGES

sp-PME100009 SIRPa page1 300.jpg

Figure 1. Human SIRPα, hFc-His Tag on SDS-PAGE under reducing condition.

elisa-PME100009 ELISA 1 SIRPa hFc His Tag

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind its native ligand Human SIRPα, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37 ng/ml.

elisa-PME100009 ELISA 2 SIRPa hFc His Tag

Figure 3. A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

sp-PME100009 SIRPa page2 300.jpg

Figure 4. Lane 1: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

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