| TARGET | |
|---|---|
| SYNONYMS |
CMT1F;CMT2E;CMTDIG;NF-L;NF68;NFL;PPP1R110 |
| DESCRIPTION |
Recombinant human NEFL(89-400) protein with N-terminal 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P07196 |
| EXPRESSION HOST |
HEK293 |
| TAG |
N-6×His Tag |
| MOLECULAR CHARACTERIZATION |
6×His tag NEFL(Gln89-Leu400) |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of His-NEFL(89-400) is approximately 35-55 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100648
Human NEFL (89-400) Protein, His Tag
Human NEFL (89-400) Protein, His Tag
PRODUCT DATA
IMAGES
Figure 1. Human NEFL(89-400) Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human NEFL (89-400) Protein, His Tag (PME100648) can bind Anti-NEFL(89-400) antibody(DM199), Rabbit mAb (DME100199) in a linear range of 0.128-80 ng/mL.
