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Dima Biotech

SKU(재고 관리 코드):PME100649

Human KRAS Protein, His Tag

Human KRAS Protein, His Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

C-K-RAS;c-Ki-ras2;CFC2;K-Ras;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KI-RAS;KRAS1;KRAS2;NS;NS3;RALD;RASK2

DESCRIPTION

Recombinant human KRAS protein with C-terminal 6×His tag

DELIVERY

In Stock

UNIPROT ID

P01116

EXPRESSION HOST

HEK293

TAG

C-6×His Tag

MOLECULAR CHARACTERIZATION

KRAS(Thr2-Cys185) 6×His tag

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 21.8 kDa after removal of the signal peptide. The apparent molecular mass of KRAS-His is approximately 15-25 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

USAGE

Research use only

IMAGES

sp-PME100649 KRAS His PAGE 300

Figure 1. Human KRAS Protein, His Tag on SDS-PAGE under reducing condition.

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