PRODUCT DATA
| Target | |
|---|---|
| Synonyms |
S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19 |
| Description |
Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal 6×His tag |
| Delivery |
In Stock |
| Uniprot ID |
P0DTC2 |
| Expression Host |
HEK293 |
| Tag |
C-6×His Tag |
| Molecular Characterization |
S protein RBD(Arg319-Phe541) 6×His tag |
| Molecular Weight |
The protein has a predicted molecular mass of 37-38 kDa after removal of the signal peptide. |
| Purity |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response. |
| Usage |
Research use only |
IMAGES
Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, His tagged protein (PME100036) can bind its native ligand Human ACE2, hFc tagged protein (PME100073) in a linear range of 1.96-31.72 ng/ml.
