| TARGET | |
|---|---|
| SYNONYMS |
CD28;Tp44 |
| DESCRIPTION |
Recombinant human CD28 protein with C-terminal mouse Fc and 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P10747 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Mouse Fc and 6×His Tag |
| MOLECULAR CHARACTERIZATION |
CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100014
Human CD28 Protein, mFc-His Tag
Human CD28 Protein, mFc-His Tag
PRODUCT DATA
IMAGES
Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-1, hFc tagged protein (PME100473) can bind Human CD28, mFc-His tagged protein (PME100014) in a linear range of 125-4000 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2, hFc tagged protein (PME100474) can bind Human CD28, mFc-His tagged protein (PME100014) in a linear range of 62.5-4000 ng/ml.
Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
