| TARGET | |
|---|---|
| SYNONYMS |
PDCD1;PD1;CD279;SLEB2 |
| DESCRIPTION |
Recombinant human PD-1 protein with C-terminal mouse Fc and 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
Q15116 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Mouse Fc and 6×His Tag |
| MOLECULAR CHARACTERIZATION |
PD-1(Phe24-Val170) mFc(Pro99-Lys330) 6×His tag |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100025
Human PD-1 Protein, mFc-His tag
Human PD-1 Protein, mFc-His tag
PRODUCT DATA
IMAGES
Figure 1. Human PD-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human PD1, mFc-His tagged protein (PME100025) can bind Anti-PD-1 Neutralizing antibody (BME100006) in a linear range of 0.24-6.49 ng/ml.
Figure 3. Flow cytometry analysis with 2μg/ml Human PD-1 Protein, mFc-His tag (PME100025) on Expi293 cells transfected with human PD-L1 (Blue histogram)or Expi293 transfected with irrelevant protein (Red histogram).
Figure 4. HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2ug/ml Human PD-1 Protein, mFc-His tag (PME100025) followed by PE-conjugated Goat anti-mouse IgG secondary antibody.
