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Dima Biotech

SKU(재고 관리 코드):PME100021

Human OX40L Protein, mFc-His tag

Human OX40L Protein, mFc-His tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

OX40L;TNFSF4;CD252;Glycoprotein Gp34;TXGP1;CD134 ligand;CD134L

DESCRIPTION

Recombinant human OX40L protein with N-terminal mouse Fc and C-terminal 6×His tag

DELIVERY

In Stock

UNIPROT ID

P23510

EXPRESSION HOST

HEK293

TAG

N-Mouse Fc and C-6×His Tag

MOLECULAR CHARACTERIZATION

mFc(Pro99-Lys330) OX40 Ligand(Gln51-Leu183) 6×His

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 44.0 kDa after removal of the signal peptide.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

USAGE

Research use only

IMAGES

sp-PME100021 SP OX40L 300

Figure 1. Human OX40 Ligand Protein, mFc-His Tag on SDS-PAGE under reducing condition.

fc-PME100021 mFc OX40 His FC Fig2

Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human OX40 (A) were surface stained with Human OX40 Ligand, mFc-His tagged protein (PME100021) 10 μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

elisa-PME100021 OX40L mFc His ELISA Fig3

Figure 3. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human OX40-L Protein, mFc-His Tag (PME100021) can bind Anti-OX40-L Rabbit mAb in a linear range of 3.2-80 ng/mL.

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