| TARGET | |
|---|---|
| SYNONYMS |
CD33;IGLEC3;p67 |
| DESCRIPTION |
Recombinant human CD33 protein with C-terminal human Fc and 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P20138 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Human Fc and 6×His Tag |
| MOLECULAR CHARACTERIZATION |
CD33(Asp18-His259) hFc(Glu99-Ala330) 6×His tag |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 53.8 kDa after removal of the signal peptide. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100039
Human CD33(18-259) Protein, hFc-His Tag
Human CD33(18-259) Protein, hFc-His Tag
PRODUCT DATA
IMAGES
Figure 1. Human CD33, hFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human CD33(18-259) Protein, hFc-His Tag(PME100039) can bind Anti-CD33 Rabbit mAb in a linear range of 3.2-16 ng/mL.
