| TARGET | |
|---|---|
| SYNONYMS |
DIF;NF-alpha;NFA;NFSF2;achexin;achectin;NFa |
| DESCRIPTION |
Recombinant human TNFa protein with C-terminal 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P01375 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-6×His Tag |
| MOLECULAR CHARACTERIZATION |
TNFa(Val77-Leu233) 6×His tag |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100076
Human TNFα Protein, His Tag
Human TNFα Protein, His Tag
PRODUCT DATA
IMAGES
Figure 1. Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human TNFa , His tagged protein (PME100076) can bind Anti-TNFa Neutralizing antibody (BME100056) in a linear range of 0.64-16 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (PME100076) can bind Anti-TNFa(Certolizumab biosimilar) mAb (BME100183) in a linear range of 0.64–16 ng/mL.
Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (PME100076) can bind Anti-TNFa(golimumab biosimilar) mAb (BME100179) in a linear range of 3.20–80 ng/mL.
