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Dima Biotech

SKU(재고 관리 코드):PME100041

Human CD30 Ligand Protein, mFc-His Tag

Human CD30 Ligand Protein, mFc-His Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

CD30-L;D153;NFSF8;D30L;D30LG;D153 antigen;D30 antigen ligand;D30 Ligand

DESCRIPTION

Recombinant human CD30 Ligand Protein with N-terminal mouse Fc and C-terminal 6×His tag

DELIVERY

In Stock

UNIPROT ID

P32971

EXPRESSION HOST

HEK293

TAG

N-Mouse Fc and C-6×His Tag

MOLECULAR CHARACTERIZATION

mFc(Pro99-Lys330) CD30 Ligand(Gln63-Asp234) 6×His

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 48.0 kDa after removal of the signal peptide.The apparent molecular mass of mFc-CD30 Ligand-His is approximately 53-70 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30 lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.

USAGE

Research use only

IMAGES

sp-PME100041 CD30L mFc His PAGE 300

Figure 1. Human CD30 Ligand Protein, mFc-His Tag on SDS-PAGE under reducing condition.

elisa-PME100041 mFc CD30L His ELISA Fig2

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Human CD30 Ligand,mFc-His tagged protein(PME100041) in a linear range of 2.56-320 ng/ml

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