| TARGET | |
|---|---|
| SYNONYMS |
EGFR;RBB;RBB1;ER1;IG61;ENA |
| DESCRIPTION |
Recombinant human EGFR Protein with C-terminal 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P00533 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-6×His Tag |
| MOLECULAR CHARACTERIZATION |
EGFR(Leu25-Ser645) 6×His tag |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide.The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100099
Human EGFR Protein, His Tag
Human EGFR Protein, His Tag
PRODUCT DATA
IMAGES
Figure 1. Human EGFR Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human EGFR, His tagged protein (PME100099) can bind Anti-EGFR Antibody (BME100034) in a linear range of 0.64-80.0 ng/ml
