| TARGET | |
|---|---|
| SYNONYMS |
CD40LG;D154;D40L;IGM1;GM;MD3;-BAM;NFSF5;RAP;p39 |
| DESCRIPTION |
Recombinant human CD40 Ligand Protein with N-terminal Human Fc tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P29965 |
| EXPRESSION HOST |
HEK293 |
| TAG |
N-Human Fc Tag |
| MOLECULAR CHARACTERIZATION |
hFc(Glu99-Ala330) CD40 Ligand(Met113-Leu261) |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100192
Human CD40 Ligand Protein, hFc Tag
Human CD40 Ligand Protein, hFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 Ligand,hFc tagged protein (PME100192) can bind Human CD40, mFc-His tagged protein (PME100015) in a linear range of 0.51-320 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40L, hFc tagged protein (PME100192) can bind Anti-CD40L Neutralizing antibody (BME100042) in a linear range of 0.64-400 ng/ml
