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Dima Biotech

SKU(재고 관리 코드):PME100929

Human CSF1 Protein, hFc Tag

Human CSF1 Protein, hFc Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

CALJA;CD73;E5NT;eN;eNT;NT;NT5;NTE

DESCRIPTION

Recombinant Human CD73 Protein with C-terminal human Fc tag

DELIVERY

In Stock

UNIPROT ID

P21589

EXPRESSION HOST

HEK293

TAG

C-Human Fc Tag

MOLECULAR CHARACTERIZATION

CD73(Trp27-Ser549) hFc(Glu99-Ala330)

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 84.1 kDa after removal of the signal peptide.The apparent molecular mass of CD73-hFc is approximately 55-100 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

USAGE

Research use only

IMAGES

sp-PME100829 CD73 hFc PAGE 300

Figure 1. Human CD73 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD73 Protein, hFc Tag (PME100829) can bind Anti-CD73 Neutralizing antibody (BME100129) in a linear range of 3.66–937.50 ng/mL.

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