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Dima Biotech

SKU(재고 관리 코드):PME100510

Human B7-H6 Protein, His Tag

Human B7-H6 Protein, His Tag

Size
Target
Uniprot ID
PRODUCT DATA
Target

Synonyms

B7-H6;CR3LG1;7 Homolog 6

Description

Recombinant Human B7-H6 Protein with C-terminal 6×His tag

Delivery

In Stock

Uniprot ID

Q68D85

Expression Host

HEK293

Tag

C-6×His Tag

Molecular Characterization

B7-H6(Asp25-Ser262) 6×His tag

Molecular Weight

The protein has a predicted molecular mass of 27.5 kDa after removal of the signal peptide.The apparent molecular mass of B7-H6-His is approximately 35-55 kDa due to glycosylation.

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage & Shipping

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Background

B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3, MIM 611550) results in natural killer (NK) cell activation and cytotoxicity.

Usage

Research use only

IMAGES

sp-PME100510 B7 H6 His PAGE 300

Figure 1. Human B7-H6 Protein, His Tag on SDS-PAGE under reducing condition.

elisa-PME100510 B7H6 His ELISA Fig2

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-H6, His tagged protein (PME100510) can bind Human NKp30, hFc tagged protein(PME100081) in a linear range of 250-2000 ng/ml.

elisa-PME100510 B7H6 His ELISA Fig3

Figure 3. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human B7-H6 Protein, His Tag(PME100510) can bind Anti-B7-H6 Rabbit mAb in a linear range of 3.2-80 ng/mL.

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