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Dima Biotech

SKU(재고 관리 코드):PME100524

Human BTLA Protein, His Tag

Human BTLA Protein, His Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

BTLA;CD272

DESCRIPTION

Recombinant human BTLA protein with C-terminal 6×His tag

DELIVERY

In Stock

UNIPROT ID

Q7Z6A9

EXPRESSION HOST

HEK293

TAG

C-6×His Tag

MOLECULAR CHARACTERIZATION

Human BTLA(Lys31-Ser150) 6×His tag

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 14.6 kDa after removal of the signal peptide.The apparent molecular mass of Human-BTLA-His is approximately 25-35 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis.

USAGE

Research use only

IMAGES

sp-PME100524 BTLA His PAGE 300

Figure 1. Human BTLA Protein, His Tag on SDS-PAGE under reducing condition.

elisa-PME100524 BTLA His ELISA Fig2

Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human BTLA , His tagged protein (PME100524) can bind Anti-BTLA Neutralizing antibody (BME100054) in a linear range of 16-80 ng/ml.

fc-PME100524 BTLA His flow转染HVEM Fig3

Figure 3. Flow cytometry analysis with 15 μg/mL Human BTLA Protein, His tag (PME100524) on Expi293 cells transfected with human HVEM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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