| TARGET | |
|---|---|
| SYNONYMS |
B7-H4;B7h.5;B7H4;B7S1;B7X;PRO1291;VCTN1 |
| DESCRIPTION |
Recombinant Human B7-H4 protein with C-terminal human Fc |
| DELIVERY |
In Stock |
| UNIPROT ID |
Q7Z7D3 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Human Fc Tag |
| MOLECULAR CHARACTERIZATION |
B7-H4 (Phe29-Ala258) hFc(Glu99-Ala330) |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide.The apparent molecular mass of B7-H4-hFc is approximately 100-130 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100053
Human B7-H4 Protein, hFc Tag
Human B7-H4 Protein, hFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human B7-H4 Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human B7-H4, hFc-His tagged protein (PME100053) can bind Anti-B7-H4 Neutralizing antibody (BME100078) in a linear range of 3.2-80 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H4 Protein, hFc Tag (PME100053) can bind Anti-B7-H4(AZD-8205 biosimilar) mAb (BME100190) in a linear range of 3.20–80 ng/mL.
Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H4 Protein, hFc Tag (PME100053) can bind Anti-B7-H4(XMT-1660 biosimilar) mAb (BME100192) in a linear range of 3.20–80 ng/mL.
