| TARGET | |
|---|---|
| SYNONYMS |
IL-5;TRF;Interleukin-5;EDF |
| DESCRIPTION |
Recombinant Human IL5 protein with C-terminal human Fc tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P05113 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Human Fc Tag |
| MOLECULAR CHARACTERIZATION |
IL5(Ile20-Ser134) hFc(Glu99-Ala330) |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide.The apparent molecular mass of IL5-hFc is approximately 35-55 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100537
Human IL5 Protein, hFc Tag
Human IL5 Protein, hFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human IL5 Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. Flow cytometry analysis with 15 μg/mL Human IL5 Protein, hFc Tag (PME100537) on Expi293 cells transfected with Human IL5RA protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL5 Protein, hFc Tag(PME100537) can bind Anti-IL5 antibody(DMC274), IgG1 Chimeric mAb in a linear range of 0.64–16 ng/mL.
