| TARGET | |
|---|---|
| SYNONYMS |
CSF-1;MCSF |
| DESCRIPTION |
Recombinant Human M-CSF Protein with C-terminal human Fc tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P09603 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Human Fc Tag |
| MOLECULAR CHARACTERIZATION |
M-CSF(Glu33-Arg255) hFc(Glu99-Ala330) |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-hFc is approximately 55-70 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100550
Human M-CSF Protein, hFc Tag
Human M-CSF Protein, hFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human M-CSF Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CSF1R, His tagged protein (PME100067) can bind Human M-CSF, hFc Tagged protein（PME100550） in a linear range of 0.32-200 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human M-CSF Protein, hFc Tag (PME100550) can bind Anti-M-CSF Neutralizing antibody (BME100104) in a linear range of 9.77–156.25 ng/mL.
Figure 4. Flow cytometry analysis with 1 μg/mL Human M-CSF Protein, hFc tag (PME100550) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
