TARGET | |
---|---|
SYNONYMS |
DCUA;DFNA69;FPH2;FPHH;Kitl;KL-1;MGF;SCF;SF;SHEP7;SLF |
DESCRIPTION |
Recombinant human SCF protein with C-terminal human Fc tag |
DELIVERY |
In Stock |
UNIPROT ID |
P21583 |
EXPRESSION HOST |
HEK293 |
TAG |
C-Human Fc Tag |
MOLECULAR CHARACTERIZATION |
SCF(Glu26-His214) hFc(Glu99-Ala330) |
MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide.The apparent molecular mass of SCF-hFc is approximately 55-75 kDa due to glycosylation. |
PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
BACKGROUND |
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
USAGE |
Research use only |
SKU(재고 관리 코드):PME100563
Human SCF Protein, hFc Tag
Human SCF Protein, hFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human SCF Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human CD117, His tagged protein (PME100519) can bind Human SCF, hFc tagged protein (PME100563) in a linear range of 3.2-400 ng/ml.