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Dima Biotech

SKU(재고 관리 코드):PME100568

Human M-CSF Protein, mFc Tag

Human M-CSF Protein, mFc Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

CSF-1;MCSF

DESCRIPTION

Recombinant Human M-CSF with C-terminal mouse Fc tag

DELIVERY

In Stock

UNIPROT ID

P09603

EXPRESSION HOST

HEK293

TAG

C-Mouse Fc Tag

MOLECULAR CHARACTERIZATION

M-CSF(Glu33-Arg255) mFc(Pro99-Lys330)

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 51.2 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-mFc is approximately 55-75 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

USAGE

Research use only

IMAGES

sp-PME100568 M CSF mFc PAGE 300

Figure 1. Human M-CSF Protein, mFc Tag on SDS-PAGE under reducing condition.

fc-PME100568 M CSF mFc flow转染CSF1R Fig2

Figure 2. Flow cytometry analysis with 1 μg/mL Human M-CSF Protein, mFc tag (PME100568) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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