TARGET | |
---|---|
SYNONYMS |
CSF-1;MCSF |
DESCRIPTION |
Recombinant Human M-CSF with C-terminal mouse Fc tag |
DELIVERY |
In Stock |
UNIPROT ID |
P09603 |
EXPRESSION HOST |
HEK293 |
TAG |
C-Mouse Fc Tag |
MOLECULAR CHARACTERIZATION |
M-CSF(Glu33-Arg255) mFc(Pro99-Lys330) |
MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 51.2 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-mFc is approximately 55-75 kDa due to glycosylation. |
PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
BACKGROUND |
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
USAGE |
Research use only |
SKU(재고 관리 코드):PME100568
Human M-CSF Protein, mFc Tag
Human M-CSF Protein, mFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human M-CSF Protein, mFc Tag on SDS-PAGE under reducing condition.
Figure 2. Flow cytometry analysis with 1 μg/mL Human M-CSF Protein, mFc tag (PME100568) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).