| TARGET | |
|---|---|
| SYNONYMS |
GIF;GLIF;MMIF |
| DESCRIPTION |
Recombinant human MIF protein with C-terminal hFc tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P14174 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Human Fc Tag |
| MOLECULAR CHARACTERIZATION |
MIF(Pro2-Ala115) hFc(Glu99-Ala330) |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 38.5 kDa after removal of the signal peptide. The apparent molecular mass of MIF-hFc is approximately 35-55 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):PME100350
Human MIF Protein, hFc Tag
Human MIF Protein, hFc Tag
PRODUCT DATA
IMAGES
Figure 1. Human MIF Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human MIF Protein, hFc Tag (PME100350) can bind Anti-MIF Neutralizing antibody (BME100098) in a linear range of 3.05–740.74 ng/mL.
