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Dima Biotech

SKU(재고 관리 코드):PME100714

Human HSD17B10 Protein, S Tag

Human HSD17B10 Protein, S Tag

Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

MHBD;Type II HADH;ERAB;HADH2;MRPP2;SCHAD;SDR5C1;XH98G2

DESCRIPTION

Recombinant human HSD17B10 protein with N-terminal S tag

DELIVERY

In Stock

UNIPROT ID

Q99714

EXPRESSION HOST

HEK293

TAG

N-S Tag

MOLECULAR CHARACTERIZATION

S tag HSD17B10(Ala2-Pro261)

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 28.5 kDa after removal of the signal peptide. The apparent molecular mass of S-HSD17B10 is approximately 35-70 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]

USAGE

Research use only

IMAGES

sp-PME100714 HSD17B10 S PAGE 300

Figure 1. Human HSD17B10 Protein, N-S Tag on SDS-PAGE under reducing condition.

sp-PME100714 HSD17B10 S PAGE2 300

Figure 2.
Gel:15%SDS-PAGE
Elution :0.1 μg
S-HSD17B10 Predicted size: 28.5kDa
Primary antibody:anti-S Tag mAb at 1:10000  dilution
Secondary  antibody:Goat anti Mouse IgG HRP at 1:10000 dilution
Exposure time :30s

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