| COMMON NAME |
BMS-734016, MDX-010, MDX-CTLA-4 |
|---|---|
| SYNONYMS |
CTLA4;CD152 |
| APPLICATIONS |
ELISA; Flow Cyt |
| RECOMMENDED DILUTIONS |
ELISA 1:5000-10000; Flow Cyt 1:100 |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| HOST SPECIES |
Homo sapiens |
| IGG TYPE |
IgG1 |
| REACTIVITY |
Human |
| TARGET | |
| UNIPROT ID |
P16410 |
| DESCRIPTION |
Anti-CTLA4 Reference mAb |
| DELIVERY |
In Stock |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):BME100022
Anti-CTLA4 Reference mAb
Anti-CTLA4 Reference mAb
PRODUCT DATA
IMAGES
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA4, mFc-His tagged protein (PME100017) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.13-16.0 ng/ml.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA4, hFc tagged protein (PME100479) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0 ng/ml. In order to specifically detect BME100022, mouse anti-human Fab-specific antibody was used as detection antibody.
Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human CTLA4 (A) were surface stained with anti-CTLA4 neutralizing antibody 1μg/ml (ipilimumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
