| TARGET | |
|---|---|
| SYNONYMS |
his tag |
| HOST SPECIES |
Rabbit |
| DESCRIPTION |
Anti-His Tag Antibody(DM1); Rabbit mAb |
| DELIVERY |
In Stock |
| IGG TYPE |
Rabbit IgG |
| CLONALITY |
Monoclonal |
| REACTIVITY |
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus. |
| APPLICATIONS |
ELISA; WB |
| RECOMMENDED DILUTIONS |
WB 1:1000 |
| PURIFICATION |
Purified from cell culture supernatant by affinity chromatography |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1); Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteinsin bacteria; insect cells; and mammalian cells. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):DME100001
Anti-His Tag Antibody(DM1), Rabbit mAb
Anti-His Tag Antibody(DM1), Rabbit mAb
PRODUCT DATA
IMAGES
Western blot analysis of his-tag fusion protein using His-Tag antibody (DME100001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000 dilution.
His-tag fusion protein : 70kDa, 20ng per lane.
Blocking buffer: 5% nonfat dry milk in TBST.
Exposure time: 1s.
