| TARGET | |
|---|---|
| SYNONYMS |
EGFR;ERBB;ERBB1;HER1;PIG61;mENA |
| HOST SPECIES |
Rabbit |
| DESCRIPTION |
Anti-EGFR antibody(DM130); Rabbit mAb |
| DELIVERY |
In Stock |
| UNIPROT ID |
P00533 |
| IGG TYPE |
Rabbit IgG |
| CLONALITY |
Monoclonal |
| REACTIVITY |
Human |
| APPLICATIONS |
ELISA; Flow Cyt |
| RECOMMENDED DILUTIONS |
ELISA 1:5000-10000; Flow Cyt 1:100 |
| PURIFICATION |
Purified from cell culture supernatant by affinity chromatography |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):DME100130
Anti-EGFR antibody(DM130), Rabbit mAb
Anti-EGFR antibody(DM130), Rabbit mAb
PRODUCT DATA
IMAGES
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human EGFR protein, His tagged protein (PME100099) can bind Rabbit anti-EGFR monoclonal antibody(clone: DM130) in a linear range of 0.06-12 ng/ml.
Figure 2. EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (DM130) on Expi293 cells transfected with human EGFR(Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram)
