TARGET | |
---|---|
SYNONYMS |
EPCAM;TACSTD1;TROP1;CD326;DIAR5;EGP2;EGP314;EGP40;ESA;GA733-2;HNPCC8;HNPCC-8;KS1:4;KSA;M4S1;MIC18;MK1 |
HOST SPECIES |
Rabbit |
DESCRIPTION |
Anti-EPCAM antibody(DM147); Rabbit mAb |
DELIVERY |
In Stock |
UNIPROT ID |
P16422 |
IGG TYPE |
Rabbit IgG |
CLONALITY |
Monoclonal |
REACTIVITY |
Human |
APPLICATIONS |
ELISA; Flow Cyt |
RECOMMENDED DILUTIONS |
ELISA 1:5000-10000; Flow Cyt 1:100 |
PURIFICATION |
Purified from cell culture supernatant by affinity chromatography |
FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
BACKGROUND |
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. |
USAGE |
Research use only |
SKU(재고 관리 코드):DME100147
Anti-EPCAM antibody(DM147), Rabbit mAb
Anti-EPCAM antibody(DM147), Rabbit mAb
PRODUCT DATA
IMAGES
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human EPCAM protein, His tagged protein (PME100068) can bind Rabbit anti-EPCAM monoclonal antibody(clone: DM147) in a linear range of 5-100 ng/ml.
Figure 2. EPCAM protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EPCAM (DM147) on Expi293 cells transfected with human EPCAM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).