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Dima Biotech

SKU(재고 관리 코드):FLP100012

Human SSTR2 full length protein-MNP

Human SSTR2 full length protein-MNP

Size
PRODUCT DATA
TARGET

SYNONYMS

SS-2-R; SS2-R; SS2R; SST2

DESCRIPTION

Human SSTR2 full length protein membrane nanoparticles (MNPs)

DELIVERY

In Stock

UNIPROT ID

P30874

EXPRESSION HOST

HEK293

PROTEIN FAMILIES

GPCR

PROTEIN PATHWAYS

Neuroactive ligand-receptor interaction

MOLECULAR WEIGHT

The human full length SSTR2 Protein has a MW of 41.2 kDa

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney.

USAGE

Research use only

IMAGES

elisa-FLP100012 SSTR2 Fig.1 Elisa 1
Figure1. Elisa plates were pre-coated with 0.5μg/per well purified human SSTR2 full length membrane nanoparticles. Serial diluted anti-SSTR2 monoclonal antibody (BME100127) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-SSTR2 monoclonal antibody binding with SSTR2 full length membrane nanoparticles is 86.2ng/ml.
fc-FLP100012 SSTR2 Fig.2 FC 1
Figure2. FACS analysis of SSTR2 MNPs
A. Negative Control 1: SSTR2 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody.
B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-SSTR2 antibody (BME100127) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
C. Negative Control 3: SSTR2 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
D. SSTR2 full length membrane nanoparticles samples were stained with anti-SSTR2 antibody (BME100127) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.

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