| TARGET | |
|---|---|
| SYNONYMS |
CC-CKR-2; CCR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R |
| DESCRIPTION |
Human CCR2 full length protein-synthetic nanodisc |
| DELIVERY |
In Stock |
| UNIPROT ID |
P41597 |
| EXPRESSION HOST |
HEK293 |
| PROTEIN FAMILIES |
Druggable Genome, Transmembrane |
| PROTEIN PATHWAYS |
Chemokine signaling pathway, Cytokine-cytokine receptor interaction |
| MOLECULAR WEIGHT |
The human full length CCR2 protein has a MW of 41.9 kDa |
| FORMULATION & RECONSTITUTION |
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein is a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The encoded protein mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This protein can also be a coreceptor with CD4 for HIV-1 infection. This gene is located in the chemokine receptor gene cluster region of chromosome 3. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):FLP100028
Human CCR2 full length protein-synthetic nanodisc
Human CCR2 full length protein-synthetic nanodisc
PRODUCT DATA
IMAGES
Figure1. Elisa plates were pre-coated with Flag Tag CCR2-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CCR2-Nanodisc is 7.62ng/ml.
Figure2. Human CCR2-Nanodisc, Flag Tag on SDS-PAGE
Figure3. WB analysis of Human CCR2-Nanodisc with anti-CCR2 monoclonal antibody (BME100087), followed by Goat Anti-Human IgG HRP at 1/5000 dilution
