| TARGET | |
|---|---|
| SYNONYMS |
ABCB1; CD243; CLCS; GP170; MDR1; p-170; P-GP; PGY1 |
| DESCRIPTION |
Human MDR-1 full length protein-synthetic nanodisc |
| DELIVERY |
In Stock |
| UNIPROT ID |
P08183 |
| EXPRESSION HOST |
HEK293 |
| PROTEIN FAMILIES |
Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| PROTEIN PATHWAYS |
ABC transporters |
| MOLECULAR WEIGHT |
The human full length MDR-1 protein has a MW of 141.5 kDa |
| FORMULATION & RECONSTITUTION |
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):FLP100029
Human MDR-1 full length protein-synthetic nanodisc
Human MDR-1 full length protein-synthetic nanodisc
PRODUCT DATA
IMAGES
Figure1. Elisa plates were pre-coated with Flag Tag MDR-1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with MDR-1-Nanodisc is 6.883ng/ml.
Figure2. Human MDR-1-Nanodisc, Flag Tag on SDS-PAGE
