| TARGET | |
|---|---|
| SYNONYMS |
GGR; GL-R; MVAH |
| DESCRIPTION |
Human GCGR full length protein-synthetic nanodisc |
| DELIVERY |
In Stock |
| UNIPROT ID |
P47871 |
| EXPRESSION HOST |
HEK293 |
| PROTEIN FAMILIES |
Druggable Genome, GPCR, Transmembrane |
| PROTEIN PATHWAYS |
Neuroactive ligand-receptor interaction |
| FORMULATION & RECONSTITUTION |
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| MOLECULAR WEIGHT |
The human full length GCGR protein has a MW of 54.0 kDa |
| BACKGROUND |
G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):FLP100085
Human GCGR full length protein-synthetic nanodisc
Human GCGR full length protein-synthetic nanodisc
PRODUCT DATA
IMAGES
Figure1. Elisa plates were pre-coated with Flag Tag GCGR-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GCGR-Nanodisc is 3.800ng/ml.
Figure2. Human GCGR-Nanodisc, Flag Tag on SDS-PAGE
