| TARGET | |
|---|---|
| SYNONYMS |
C5A; C5AR; C5R1; CD88 |
| DESCRIPTION |
Human C5AR1 full length protein-synthetic nanodisc |
| DELIVERY |
In Stock |
| UNIPROT ID |
P21730 |
| EXPRESSION HOST |
HEK293 |
| PROTEIN FAMILIES |
Druggable Genome, GPCR, Transmembrane |
| PROTEIN PATHWAYS |
Complement and coagulation cascades, Neuroactive ligand-receptor interaction |
| FORMULATION & RECONSTITUTION |
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| MOLECULAR WEIGHT |
The human full length C5AR1 protein has a MW of 39.3 kDa |
| BACKGROUND |
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):FLP100086
Human C5AR1 full length protein-synthetic nanodisc
Human C5AR1 full length protein-synthetic nanodisc
PRODUCT DATA
IMAGES
Figure1. Elisa plates were pre-coated with Flag Tag C5AR1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with C5AR1-Nanodisc is 4.088ng/ml.
Figure2. Human C5AR1-Nanodisc, Flag Tag on SDS-PAGE
