| TARGET | |
|---|---|
| SYNONYMS |
LDLCQ7; NPC11L1; SLC65A2 |
| DESCRIPTION |
Human NPC1L1 full length protein-synthetic nanodisc |
| DELIVERY |
In Stock |
| UNIPROT ID |
Q9UHC9 |
| EXPRESSION HOST |
HEK293 |
| PROTEIN FAMILIES |
Druggable Genome, Transmembrane |
| PROTEIN PATHWAYS |
—— |
| FORMULATION & RECONSTITUTION |
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| MOLECULAR WEIGHT |
The human full length NPC1L1 protein has a MW of 148.7 kDa |
| BACKGROUND |
The protein is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| USAGE |
Research use only |
Dima Biotech
SKU(재고 관리 코드):FLP100089
Human NPC1L1 full length protein-synthetic nanodisc
Human NPC1L1 full length protein-synthetic nanodisc
PRODUCT DATA
IMAGES
Figure1. Elisa plates were pre-coated with Flag Tag NPC1L1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with NPC1L1-Nanodisc is 4.526ng/ml.
Figure2. Human NPC1L1-Nanodisc, Flag Tag on SDS-PAGE
