anti- BrdU antibody

Study BrdU (5-Bromo-2'-deoxyuridine) incorporation with our Anti-BrdU Antibody, available in a 100µg size and featuring a monoclonal design for enhanced specificity. This high-quality antibody is your essential tool for detecting and quantifying BrdU-labeled DNA in cellular proliferation and DNA synthesis assays.

Designed for precision and reliability, this monoclonal antibody ensures exceptional sensitivity in applications such as immunofluorescence, flow cytometry, and cell cycle analysis. Whether you're investigating cell proliferation, DNA replication, or S-phase labeling, this antibody delivers accurate and reproducible results.

BrdU is a thymidine analog commonly used to label and trace newly synthesized DNA in proliferating cells. It is widely employed in studies related to cell division, tissue regeneration, and cancer research.

With our Anti-BrdU Antibody, you can precisely assess BrdU incorporation, monitor cell cycle dynamics, and advance research in cell biology and cancer biology. Elevate your research in DNA replication and cell proliferation with this monoclonal antibody.

Product Name

BrdU antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

IMMUNOGEN INFORMATION

Immunogen

compound

Synonyms

5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR

APPLICATION

Tested Application

ELISA, IF, IHC

Recommended Dilution

IHC: 1:200-1:1000; IF: 1:50-1:500

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