anti- ZAK antibody

PRODUCT INFORMATION

Product Name

ZAK antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

IMMUNOGEN INFORMATION

Immunogen

sterile alpha motif and leucine zipper containing kinase AZK

Synonyms

AZK, HCCS 4, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

Observed MW

55 kDa, 100 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:20 - 1:50

UNIPROT INFORMATION

UniProt ID

Q9NYL2

Immunohistochemistry of paraffin-embedded human colon carcinoma slide using FNab09581( ZAK Antibody) at dilution of 1:50

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09581(ZAK antibody) at dilution of 1:1000