anti- DNA2 antibody

The anti-DNA2 antibody, available in a 100µg size, is a valuable research tool for scientists studying DNA2, a multifunctional nuclease/helicase involved in DNA replication, repair, and maintenance. This antibody offers high specificity and sensitivity, making it suitable for various applications, including immunohistochemistry and Western blotting.

DNA2 is known for its critical roles in DNA metabolism and genome stability. Researchers can employ this antibody to investigate the expression patterns and functions of DNA2, contributing to our understanding of its roles in maintaining genomic integrity.

Rigorous quality control ensures the reliability and consistency of results, providing researchers with the confidence to advance their scientific investigations effectively. With 100µg of product per vial, it offers an ample supply for multiple experiments, ensuring cost-effectiveness and experimental flexibility.

In summary, the anti-DNA2 antibody is a high-quality reagent that empowers researchers to investigate the roles of DNA2 in DNA replication and repair. By studying DNA2, researchers can contribute to our understanding of essential cellular processes and their implications in health and disease.

Product Name

DNA2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

IMMUNOGEN INFORMATION

Immunogen

DNA replication helicase 2 homolog

Synonyms

DNA2, DNA2L, KIAA0083

Observed MW

127 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P51530

IMAGES