anti- BRCA2 antibody

Uncover the secrets of BRCA2 (Breast Cancer Susceptibility Gene 2) with our Anti-BRCA2 Antibody, available in a 100µg size and offered as a polyclonal antibody. This high-quality antibody is your key to investigating the multifaceted roles of BRCA2 in DNA repair, genome stability, and breast cancer susceptibility.

Engineered for precision and versatility, this polyclonal antibody is suitable for applications such as immunohistochemistry, immunoprecipitation, and fluorescence microscopy. Whether you're unraveling BRCA2's involvement in homologous recombination, cell cycle regulation, or ovarian cancer predisposition, this antibody empowers your research with accuracy and sensitivity.

BRCA2 is a tumor suppressor gene known for its essential role in homologous recombination-mediated DNA repair, safeguarding genomic integrity. Mutations in BRCA2 are associated with a heightened risk of breast, ovarian, and other cancers.

With our Anti-BRCA2 Antibody, you can study BRCA2's functionality, investigate its interactions with DNA repair machinery, and potentially advance research in cancer genetics and therapy. Elevate your research in DNA repair mechanisms and hereditary cancer syndromes with this precision antibody.

Product Name

BRCA2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA(ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A(RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex(transcription and export complex 2) subunits PCID2 and SHFM1/DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination(PubMed:24896180).

IMMUNOGEN INFORMATION

Immunogen

breast cancer 2, early onset

Synonyms

FACD, FANCD1, breast cancer 2, early onset, BROVCA2

Observed MW

384kd

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:200-1:1000

UNIPROT INFORMATION

UniProt ID

P51587

IMAGES

A549 cells were subjected to SDS PAGE followed by western blot with FNab10454(BRCA2 antibody) at dilution of 1:800