anti-Pan-AKT antibody

Product Name

Pan-AKT antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.

IMMUNOGEN INFORMATION

Immunogen

human AKT1/AKT2/AKT3

Synonyms

AKT1/AKT2/AKT3

Observed MW

60 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:200-1:800; IHC: 1:20-1:100; IF: 1:20-1:100; IP: 1:20-1:100

UNIPROT INFORMATION

UniProt ID

P31749/P31751/Q9Y243

IMAGES

Immunohistochemistry of paraffin-embedded rat kidney tissue slide using FNab10483( Pan-AKT Antibody) at dilution of 1:50

Immunofluorescent analysis of U2OS cells using FNab10483( Pan-AKT Antibody) at dilution of 1:50.

A549 cells were subjected to SDS PAGE followed by western blot with FNab10483( Pan-AKT Antibody) at dilution of 1:500