anti-GRP78/BIP antibody

Product Name

GRP78/BIP antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

IMMUNOGEN INFORMATION

Immunogen

Endoplasmic reticulum chaperone BiP

Synonyms

HSPA5, BIP, GRP78, HEL-S-89n, MIF2

Observed MW

70-78 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:2000-1:20000; IHC: 1:50-1:200; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P11021

IMAGES

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab10492(GRP78/BIP antibody) at dilution of 1:50

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab10492(GRP78/BIP antibody) at dilution of 1:5000