anti-CHAF1b antibody

Product Name

CHAF1b antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

IMMUNOGEN INFORMATION

Immunogen

Chromatin assembly factor 1 subunit B

Synonyms

CHAF1B, CAF-1, CAF-IP60, CAF1

Observed MW

70 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

Q13112

IMAGES

Immunohistochemistry of paraffin-embedded mouse spleen slide using FNab10505(CHAF1b antibody) at dilution of 1:50

Immunofluorescent analysis of MCF-7 cells using FNab10505(CHAF1b antibody) at dilution of 1:50.

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab10505(CHAF1b antibody) at dilution of 1:1000