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SKU(재고 관리 코드):EH13197
Human TRIM25(E3 ubiquitin/ISG15 ligase TRIM25)ELISA Kit
Human TRIM25(E3 ubiquitin/ISG15 ligase TRIM25)ELISA Kit
Product Name
Human TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25)ELISA Kit
Alias
E3 ubiquitin/ISG15 ligase TRIM25 ELISA Kit, Estrogen-responsive finger protein ELISA Kit, RING finger protein 147 ELISA Kit, RING-type E3 ubiquitin transferase ELISA Kit, RING-type E3 ubiquitin transferase TRIM25 ELISA Kit, Tripartite motif-containing protein 25 ELISA Kit, Ubiquitin/ISG15-conjugating enzyme TRIM25 ELISA Kit, Zinc finger protein 147 ELISA Kit, EFP ELISA Kit, RNF147 ELISA Kit, ZNF147 ELISA Kit, TRIM25 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TRIM25 ELISA Kit allows for the in vitro quantitative determination of TRIM25 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TRIM25 and the recovery rates were calculated by comparing the measured value to the expected amount of TRIM25 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
95-101 |
99 |
|
EDTA plasma(n=5) |
88-104 |
97 |
|
heparin plasma(n=5) |
92-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRIM25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
90-102% |
87-104% |
|
EDTA plasma(n=5) |
85-98% |
84-101% |
83-96% |
|
heparin plasma(n=5) |
81-93% |
89-99% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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