FineTest
SKU(재고 관리 코드):EU2612
15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Product Name
15-HETE (15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Alias
15-HETE ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
15-HETE ELISA Kit allows for the in vitro quantitative determination of 15-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 15-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 15-HETE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-94 |
91 |
|
EDTA plasma(n=5) |
91-104 |
95 |
|
heparin plasma(n=5) |
91-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-99% |
86-104% |
91-105% |
|
EDTA plasma(n=5) |
89-101% |
89-100% |
90-100% |
|
heparin plasma(n=5) |
90-94% |
91-95% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
