FineTest
SKU(재고 관리 코드):EH10021
Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit
Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit
Product Name
Human MDM2(E3 ubiquitin-protein ligase Mdm2) ELISA Kit
Alias
E3 ubiquitin-protein ligase Mdm2 ELISA Kit, Double minute 2 protein ELISA Kit, Hdm2 ELISA Kit, Oncoprotein Mdm2 ELISA Kit, RING-type E3 ubiquitin transferase Mdm2 ELISA Kit, p53-binding protein Mdm2 ELISA Kit, MDM2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MDM2 ELISA Kit allows for the in vitro quantitative determination of MDM2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MDM2 and the recovery rates were calculated by comparing the measured value to the expected amount of MDM2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
95-105 |
99 |
|
EDTA plasma(n=5) |
95-105 |
99 |
|
heparin plasma(n=5) |
96-103 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MDM2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-103% |
89-99% |
90-104% |
|
EDTA plasma(n=5) |
89-99% |
89-95% |
89-100% |
|
heparin plasma(n=5) |
90-99% |
90-97% |
92-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
